Veronica Taylor ... Ash Ketchum (voice: English version)
Philip Bartlett ... Mewtwo (voice: English version)
Rachael Lillis ... Misty / Jessie / Wigglytuff (voice: English version)
Eric Stuart ... Brock / James / Squirtle (voice: English version)
Madeleine Blaustein ... Meowth / Head Scientist / Pirate Trainer (voice: English version) (as Addie Blaustein)
Ikue Ôtani ... Pikachu (voice) (as Ikue Otani)
Ted Lewis ... Giovanni / Corey - Male Trainer (voice: English version) (as Ed Paul)
Jimmy Zoppi ... Fergus - Water Trainer (voice: English version)
Michael Haigney ... Psyduck / Venusaur / Blastoise / Dewgong (voice: English version)
Kayzie Rogers ... Vaporeon / Vileplume (voice: English version)
Rodger Parsons ... Narrator (voice: English version) (as Ken Gates)
Lee Quick ... Officer Jenny (voice: English version)
Lisa Ortiz ... Neesha (voice: English version)
Amy Birnbaum ... (voice: English version)
Megan Hollingshead ... Nurse Joy (voice: English version)
Eric Grossfeld ... (voice: English version)
Tara Sands ... Bulbasaur / Vulpix / Ninetales (voice: English version) (as Tara Jayne)
Satomi Koorogi ... Togepi (voice) (uncredited)
Shinichirô Miki ... Charizard / Staryu (voice) (uncredited)
Kôichi Yamadera ... Mew (voice) (uncredited)

When a group of scientists are offered funding into genetic research if they agree to try and clone the greatest ever Pokémon, Mew, the end result is success and Mewtwo is born. However Mewtwo is bitter about his purpose in life and kills his masters. In order to become the greatest he throws open a challenge to the world to battle him and his Pokémon. Ash and his friends are one of the few groups of trainers who pass the first test and prepare for battle. However they soon find out about further cloning and Mewtwo's ultimate plan for the earth.

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